Monday, November 25, 2019

Verbs Followed by Infinitive

Verbs Followed by Infinitive Many verbs are followed immediately by the infinitive form of the verb. Other verbs are followed by the gerund form of the verb. Finally, other verbs are followed by a noun, noun phrase or pronoun and then the infinitive. All of these verbs follow no specific rules and must be memorized. You can practice your knowledge once youve reviewed this sheet, as well as the other verb pattern reference lists by taking these quizzes: Verb Form - Gerund or Infinitive Quiz 1 Verb Form - Gerund or Infinitive Quiz 2 Gerund or Infinitive? An Interactive Reference Chart and Quiz The following list provides verbs that are immediately followed by the infinitive form of another verb (verb to do). Each verb followed by the infinitive is followed by two example sentences to provide context. affordI cant afford to go on vacation this summer.Can you afford to buy that sweater?agreeI agreed to help him with the problem.Do you think he would agree to take the test again?appearHe appears to think Im crazy!They appear to be available tomorrow.arrangeI arranged to spend the week in New York.Mary arranges to meet everyone each time.askShe asked to do the job.Franklin will ask to be promoted.begShelley begged to be released as soon as possible.The minister begged to donate as much as possible.careDo you care to spend some time with me?Tom doesnt care to ask any more questions.claimconsentWe consented to adopt the measure in the next year.Sherry will consent to marry you. Im sure!dareThose kids wont dare to break into that house.She often dares to break convention.decideIm going to decide to appoint the teacher next week.Mary and Jennifer decided to purchase an old house to fix up.demandThe protesters demanded to see the president about the economy.The client demanded to speak wi th his lawyer before making a statement. deserveI think Jane deserves to get the promotion.Our boss deserves to be fired!expectTom expects to finish the job soon.The students expect to receive their grades before the end of the day.failSusan never fails to mention that she knows the president personally.You shouldnt fail to mail in the form by the end of the week.forget - NOTE: This verb can also be followed by the gerund with a change in meaning.I think Peter forgot to lock the door before he left home.We seldom forget to do our homework, but last week was an exception.hesitateI hesitate to mention this, but dont you think ...Doug hesitated to tell us about his plan.hopeI hope to see you soon!He had hoped to have more success before he lost the election.learnHave you ever learned to speak another language?Our cousins are going to learn to mountain climb on vacation.manageTed managed to get his work done on time.Do you think well manage to persuade Susan to come with us?meanTim certainly meant to finish the job on time.They mean to do business here in town. needMy daughter needs to finish her homework before she can come out and play.They needed to fill out a number of forms in order to purchase the house.offerJason offered to give Tim a hand with his homework.She offers to help students whenever they have a question.planOur class plans to put on a play next semester.Im planning to visit you when Im in New York next month.prepareOur teachers are preparing to give us a test today.The politicians prepared to debate the issues on television.pretendI think he is pretending to be interested in the subject.She pretended to enjoy the meal, even though she didnt think it was good.promiseYes, I promise to marry you!Our coach promised to give us next Friday off if we win the game.refuseThe students refused to quiet down at the assembly.I think you should refuse to do that job.regret - NOTE: This verb can also be followed by the gerund with a change in meaning.I regret to tell you that it is not possible.The officer regretted to inform the citizen s of the horrific facts about the case. remember - NOTE: This verb can also be followed by the gerund with a change in meaning.Did you remember to lock the doors?I hope Frank remembered to telephone Peter about the appointment.seemIt seems to be a beautiful day outside!Did he seem to be nervous?struggleThe boys struggled to understand the concepts presented in the lesson.I sometimes struggle to stay concentrated when Im on the job.swearDo you swear, to tell the truth, the whole truth, and nothing but the truth?Alice swore to help in any way possible.threatenChris threatened to call the police.The owner will threaten to kick you out if you dont stop making noise.volunteerId like to volunteer to judge the competition.Sarah volunteered to take Jim to the piano lesson.waitIm waiting to hear from Tom.She waited to eat until he arrived.wantJack wants to help everyone with the new concepts.The principal wanted to put on a teacher workshop.wishI wish to see you soon.Franklin wished to come and visit last month. More Verb Pattern Reference Lists: Verbs followed by the gerund - Verb Ing Verbs followed by a (pro)noun plus the infinitive - Verb (Pro)Noun Infinitive Verbs followed by the infinitive - Verb Infinitive

Friday, November 22, 2019

Great Wall Golf & Country Club Case Study Example | Topics and Well Written Essays - 1250 words

Great Wall Golf & Country Club - Case Study Example The goal of Great Wall Golf & Country Club was not only to maintain its status but to be the best in Asia. From the case, we find that majority of senior managers were of Chinese origin who were recruited from the hospitality industry of Singapore and Hong Kong. When the Chinese market was at it’s over saturation level, the employees from the Mainland China were needed to take up the sport in order to continue the pace of Golf Course Development. Also, in order to increase the efficiency of the operations and developing specific skills, Great Wall Golf & Country Club organized itself with the help of HRM Department. The HR Department was used as a means of change agent, administrative expert, strategic partner and the champion of the employee in order to deliver the value. Recruitment & Retention: In order to meet the growing demand of Golfing facilities in China, 2000 employees were recruited by placing ads on the newspaper. The employees were selected on the basis of their e xperience and interpersonal skills accessed during an interview by HR Department. Due to the migrant nature of the employees, staffing was considered as a challenge for many businesses. In spite of being paid high, many employees resigned from the job after 18 to 24 months either due to financial reasons or because they wanted to be with their families. The Great Wall Golf & Country Club had the ability to retain the staffs due to their long-standing relations with the members. Food & Beverage, Caddies & Membership services, Golf Club reception were the departments where the long term services would initiate towards increasing the service standards of the customer thereby enabling The Great Wall Golf & Country Club to obtain its strategic objective, i.e., provide services of high quality. Education and Training & Development: The goal of Great Wall Golf & Country Club was to give services of high quality to its 2000 employees. The successful businessmen and government officials pref erred a high quality service. Delivery of high quality service was a challenging task in China which could be achieved by educating and training the employees. In many service industries, the employees were given the same pay cheque without considering the quality of the work. Human Resource Department provided language and practical training to the employees, generating awareness regarding the service and the mission, increasing the confidence level of the employees and also paying them according to the quality of the work performed in order to enhance their performance level for delivering better services. Therefore, it can be observed that the HRM practices in Great Wall Golf & Country Club fits together strategically. The strategies not only enabled the organization to attract employees from different regions but also retain them, as provided facilities at Great Wall were considerably more attractive compared to the industrial settings. The employees, especially who were migrate d, felt more confident as their fundamental needs were taken care of by the HRM practices. 2.0. How Do the HRM Practices at Great Wall Support Great Wall’s Strategy? Great Wall’s strategy has been to be world class in terms of provided services and facilities and also to be ‘among the best in Asia’. Great Wall’s prime emphasis was related towards quality, profit as well as innovation. The other aspects

Wednesday, November 20, 2019

Difference between Leaders and Managers Assignment - 13

Difference between Leaders and Managers - Assignment Example Following these theories in leadership, leaders should depict voluntary action; this is the difference between leaders and managers. Leaders in the article are acting like managers, whose role is to control the employees. They fail in their roles as they are supposed to listen to their needs and look for a way forward to create an admirable working environment. The success of leaders has a different interpretation, as it is governed by many variables; this includes the leadership styles, aspects of the situation, and quality of followers (Bertocci 22). This means that they have to create a basis to enhance their relationship with employees. Â  In the recent business world, there is a need for an integrated education system, which will produce effective people in the job market. This is the main objective Ohio States studies. Through this kind of initiatives, business leaders in China will be in a position to relate professionally with both customers and employees, which is the most prominent object in any organization. With this, industries will eliminate strikes enhance increase their productivity (Bertocci 45).

Monday, November 18, 2019

Book Review How Did That Happen by Roger Connors and Tom Smith 2009 Coursework

Book Review How Did That Happen by Roger Connors and Tom Smith 2009 - Coursework Example Something we go to when we are very close to pulling all our hair out from sheer frustration. Summary In most cases, we just need a surefire way to fix our problem, something that will guide us to the step of recovery, to save us from what has gone incredibly wrong and probably made us lose more than just our money. In such situations, more than the financial losses, we are worried about the amount of time, effort and energy we had put into the project. More often than not, we stress over our projects and spend sleepless nights wondering if our plan will be accepted by our targets and if we would achieve the success we had set out to. And when our project fails even a little bit, we can all but hang our heads in shame and disappointment. In How did that happen Roger Connors and Tom Smith address such situations. In the scene of the global economic crisis and where it was born, all managers have at some point or the other asked how all their plans went awry and how everything they had done was washed down the drain. But it’s not often that one can get answers to such questions. For one, the answer to every how did that happen is completely different. And another, no one will take the onus to explain to you what went wrong when you executed a particular plan. It is almost always up to you, the designer of the plan, to figure out what went wrong in your master plan, and where. In this day and age, we have the option to outsource our problems to consultants. They do an in-depth study of our plans and arrive at what went wrong and give us suggestions on how to rectify it. â€Å"Successfully holding others accountable to deliver on expectations, and doing it in a way that make others feel good about it, requires real effort and skill.† (Connors & Smith, 2009, pg.15-16). In spite of this, organizations may find it quite embarrassing to hand over their failures to random strangers. It makes the most sense to find the loopholes in the plan we have worked s o hard on. Review They say prevention is better than cure. But when the disease strikes, we cannot sob over not having taken the prevention. It is time for action. However, in most cases, when such crises strike, we have no clue where to begin to clean up the mess. We know that we have to do something to save our company from drowning, to keep ourselves afloat, but when disaster strikes, we lose our cool in all the panic. We need guidance, someone to tell us exactly what to do, when to do and how to do it. The only problem is, no one ever wants to take the responsibility to be that someone, probably out of fear of flopping out again. This is when smart people decided to write about foreseen crises when they were in a sober state of mind, so that when the problem showed its ugly face, people would have somewhere to turn to - a guide, a direction to move in to rectify what has gone horribly wrong. In How did that happen, Connors and Smith have defined in a very simple and comprehensib le manner what one must do in case of such outbreaks. Rather than giving us vague illustrations and long, complex instructions that no one will understand and confusing us even more, they have managed to break down the whole concept and give us step-by-step instructions that are definite, as opposed to the ambiguity one usually finds in such books. This may be the most important factor of this book, that even in spite of being a self-help book, it actually

Saturday, November 16, 2019

J. David Bamberger: A Biography

J. David Bamberger: A Biography A  champion  of  land  stewardship  and  habitat  restoration J. David Bamberger has become an internationally known and highly respected conservationist who has dedicated his life work to protecting the environment while using its resources. He has transformed a once blighted.useless property into a beautiful sanctuary that serves as a model for others interested in habitat restoration. He has made strides in protecting and growing certain endangered species populations which have made positive impacts around the world. Bamberger shares the knowledge he has gained throughout his life by teaching others through trainings and workshops. Bamberger grew up in rural Ohio during the Great Depression and World War II. He had always admired the Amish for their ability to live in harmony with the land. (Greene, 2007) His father was a farmer so he learned to grow food at a young age. (Mcleod, 2001) His mother gave him a book titlePleasant Valley, written by Louis Bromfield, when he was growing up. Bromfield was an American advocate for land restoration and he wrote about his ideas in that book. Bamberger once said if I ever make money I want to do what Bromfield did,(Greene,  2007) referring to his work in habitat restoration. According to Goodwyn, (2010), Bamberger began working in Ohio as a door to door vacuum cleaner salesman in the 1940s. He received a Bachelor of Science Degree from Kent State University in 1950 before he moved to Texas where he continued to sell vacuum cleaners. He worked long hard hours with few days off to make as much money as he could, as he was paid by commission. Although his family was disappointed in his initial career choice according to Goodwyn (2010), he was able to bank half of a million dollars over a ten year period. Then he met another vacuum salesman named Bill Church who was trying to expand his fried chicken restaurant but needed some financial backing. Bamberger used some of his fortune to invest into the company known as Churchs Fried Chicken and became a partner in that business. By the  late 1960s the restaurant expanded across Texas making them both very wealthy. Bamberger used the wealth he had worked for to fulfill a lifetime dream in habitat restoration. Goodwyn (2010) reported while realtors were trying to show him properties with air strips and nice houses, he told them he wanted something nobody else wants. He ended up purchasing a 5500 acre ranch that was full of juniper plants which choke out other plants by spreading and taking over the landscape so nothing else can grow, reported in the article Restoring the Range. The land was overgrazed from years of misuse and there was little topsoil which had been blown away by wind due to the absence of plant root systems which would have held moisture in the soil. The creeks on the property were mostly dried up and not supporting the habitat. The animals that lived on the ranch were undernourished as well due to the lack of food in the area. Bamberger worked for decades to restore the ecological balance of the property.   He cleared the land of Juniper, carved plateaus into the hillsides to hold rain water, planted grass to hold the soil in place and to absorb the water into the soil. (Goodwyn, 2010). He changed and improved the water distribution across the property and planted trees and wildflowers according to the article Restoring the Range. Mcleod (2001) cited the incredible work he has accomplished on his property is evident by simply looking at his fence line. On one side of the fence, Junipers are still ravaging the landscape while on his side of the fence there are fields of grass along with a diversity of many other plants. The wildlife on the property have prospered on the revitalized ground according to the research. Bamberger said there were initially 48 species of birds when he first acquired the property but that number has climbed to over 219 species. (Restoring the Range, 2012) At the beginning the best deer harvested weighed 55 pounds after field dressing. (Mcleod, 2001) Now the average weight is 105 pounds. The ranch is also a working farm that raises and sells livestock. According to Goodwyn (2010), he used one square mile of his property to provide a  habitat for a highly endangered Scimitar-homed Oryx, an antelope that had disappeared from the African Sahara. The animal is currently extinct in the wild but still bred in captivity. The ranch is now home to a large herd of thriving Oryx and breeding aged males are regularly traded with  zoos and other animal preserves to preserve genetic diversity. He carved a three dome cave into a hillside on his property in 1998 and lined the ceiling wi th gunite, a mixture of cement, sand, and water that is generally sprayed into tunnels to line them with a hard dense layer. This is now  home to more than 400,000 Mexican Free-Tailed Bats.   Bamberger ranch has been one of the largest habitat restorations in Texas.   His work has not only improved the quality oflife for the 5500 acres and species living there, but has served as a model for others to follow. Goodwyn (2010) acknowledged he has improved the water quality of the water on his land which positively effects everyone downstream of his property. Bamberger hosts research on grasslands and range management at the ranch. He holds conferences and educational workshops on habitat restoration for students, landowners,  scientists, and conservation agencies.   He has earned dozens of awards for his work on the ranch and in the community. J. David Bambergers life journey is an inspiration to others. His inspiration came from a book his mother bought him. He worked hard, saved money, and fulfilled his dream. He found the worst piece of property he could find and he was able to transform it into a model for land stewardship. The refurbished property now boasts healthy water and vegetation. His work didnt stop there, as he added endangered species to the property,   as well as helped the species that  were already there to prosper. Then, he continued to help the enviromnent by passing on the knowledge he has gained through his experience to others by hosting training and workshops. He has shown that everyone can make a difference if they set their mind to it. Works Cited Around the Nation. Texas Rancher An Unlikely Environmentalist, Heard on All Things  Considered, by Wade Goodwyn, 2 February 2010 http://www.npr.org/templates/story/story.php?storylcl=12306868 l The Austin Chronicle, Day Trips, J. David Bamberger has taken a slice of the Hill Country and restored it to pristine beauty, by Gerald E. Mcleod, 12   October   2001  http://www.austinchronicle.com/columns/2001-10-12/8325 l/ Making a Difference: Restoring the Range, posted 29 May 2012 https ://hmclecozine.com/2012/05/29/restoring-the-range/ Water From Stone, The Story of Selah, Bamberger Ranch Preserve, by Jeffrey Greene, 26 March  2007

Wednesday, November 13, 2019

Band of Brothers by Stephen Ambrose :: American History

Band of Brothers by Stephen Ambrose I. Authors Background Stephen Ambrose was born in 1936 and grew up in Whitewater, Wisconsin, a small town where his father was the M.D. At the University of Wisconsin, he started as a pre-med, but inspired by a great professor he changed his major to History. After getting his M.A. degree at Louisiana State University, he returned to the University of Wisconsin to complete a Ph.D. Ambrose began teaching at the University of New Orleans. He started as a Civil War historian but changed to political history after President Eisenhower asked him to become his biographer. Since then, Ambrose has written more than twenty books. Among his best sellers are D-Day, Citizen Soldiers, Band of Brothers, Undaunted Courage and Nothing like it in the World. He was also a consultant for Steven Spielberg’s movie Saving Private Ryan. He is a retired Professor of History. Ambrose is now the director of the Eisenhower Center in New Orleans and is the founder of the National D-Day Museum. He is also a contributing editor of the Quarterly Journal of Military History. II. Synopsis Band of Brothers is a fascinating book that captures moments lived by soldiers during World War II. It specifically relates to the History of a small unit of paratroopers known as Easy Company, 506 Regiment, 101st Airborne. It is a story that follows the company from its inception to the capture of Hitler’s nest. It begins with the training of these soldiers at Camp Toccoa, Georgia. The 140 members of easy company who were young men from different social levels were physically and mentally trained. This particular company had an extremely harsh training, but many believe it is because of this training that they were considered as one of the best rifle companies in the army. Their trainer was Captain Sobel who they disliked but was later replaced with Lieutenant Winters. Two of the many things these soldiers learned were brotherhood and leadership. The first drop done by the paratroopers was on June 6, 1944 in Normandy. This drop did not result as planned. Planes were flying at a high velocity and at a short distance from the ground. Therefore one of the planes got hit. They arrived at Carentan which they captured and flew back to Aldbourne. Their second drop was on September 17, 1944 in Holland. This time it was perfectly done.

Monday, November 11, 2019

Evaluation of Genetic Diversity of Golden Apple Snail, Pomacea Canaliculata

ABSTRACT Genetics is a trend these days especially that, DNA barcoding has been developed. DNA barcoding is an important tool in categorizing the taxa of different species and it tells so much about the species’ traits, including genetic diversity. The Pomacea canaliculata was introduced in different parts of Asia and had been an invasive species and a pest in different ecosystems ever since the introduction. In understanding this species of snails, samples were collected, DNA’s were extracted, undergone PCR and electrophoresis, and was sequenced and analyzed.The analysis was qualitative In the Philippines while quantitative in China. In the Philippines, the cytochrome oxidase subunit 1 (COI) genes was used and compared among the species collected and when it was sequenced, it showed differences due to localized gap, mismatch and non-correspondence of bases. And in China, the diversity was analyzed by means of Nei’s gene diversity, Shannon’s information in dex, percentage of polymorphic bands (PPB) an AMOVA anlysis. Both the quantitative and qualitative showed that there was diversification within and among the populations of these snails.INTRODUCTION Genetics is the study of the genes, and the heredity and variation of individuals. Understanding the genome, which is the complete set of chromosomes or the entire genotype of an individual, is important because it helps in the taxonomy of species, especially nowadays wherein advancements in science needs more specific information, and that basing on morphological features is not enough. Life is specified by genomes which contain all the biological information which is encoded in its deoxyribonucleic acid (DNA) and divided into units or the genes.The genes are the blueprint for life because it is the particulate determiner of hereditary traits. Hence, DNA barcoding became a trend for scientists and researchers for the understanding of the different variations in the traits of different o rganisms. The golden apple snails (Pomacea canaliculata) originated from the South America, Central America, the West Indies and the Southern USA (Pain 1972) and was spread in the past decades to the different parts of Southeast Asia, namely Philippines, China, Thailand, Cambodia, Hong Kong, Indonesia and Japan.The introduction of the P. canaliculata without prior studies caused damages to the different plants and it became an invasive species which resulted to becoming pests to humans and competitors to other local snails, example of which are those from the genus Pili. The P. canaliculata was observed to have different growth and reproduction in different parts of Asia, together with their external characteristics due to the different habitats and environmental conditions (Keawjam, 1986 and 1987), therefore there is the possibility to misidentify two sympatric species as one.On the other hand allopatric populations inhabiting different habitats may show ecomorphological variations and questionable species status and it was also suggested that the golden apple snails had high adaptability hence it was easier for them to form new populations (Dong et al. 2011). The understanding of the genomes of different species is a trend for scientists these days but the information about the different mollusks is still limited. The basic information on the number of species and/or population is of help for conservation programs (Carvalho and Hauser, 1994) and for building appropriate management schemes.In contribution, the studies aims are to evaluate the genetic diversity of the golden apple snail population in Asia, namely, Philippines and China via molecularly characterizing the P. canaliculata and to find different ways of analyzing the gathered data from the sequenced DNA of the said species. REVIEW OF RELATED LITERATURE Genetic diversity (Reed 2005) The significance of genetic diversity arose from two necessities: genetic diversity is required for populations to evo lve in response to environmental changes and heterozygosity levels are linked directly to reduce population fitness via inbreeding depression.The amount of genetic variation a population contains is predicted to correlate with current fitness and, in the case of heritabilities (which can remain high or even increase despite severe reductions in population size) with evolutionary potential. This correlation between fitness and levels of genetic variation, however, may be weak or nonexistent due to the neutrality of molecular markers used in estimating heterozygosity, nonadditive genetic variation and the purging of deleterious alleles because of increased selection against homozygotes.There is a body of literature that suggests that allozyme heterozygosity is a good measure of population fitness and adaptive potential. Others caution though that such molecular genetic data generally reflect only a small portion of genome and thus may not be indicator of adaptive genetic differences. But molecular markers may be useful for assessing the extent of genetic drift. Moreover, deleterious alleles, in mutation-selection balance, are responsible for at least half of the genetic variation in fitness.Selection has the tendency to purge the population of the deleterious recessive alleles which in theory creates inbred populations with a higher fitness than their outbreed progenitor. In other words, inbred populations with less genetic diversity would have higher fitness if the population is not kept small enough for a long enough to allow the fixation of deleterious alleles to occur. Fitness and future adaptability are reduced in smaller populations of plants and animals due to drift and inbreeding depression.Commonly used surrogates for fitness such as heritabilities, heterozygosity, and population size, significantly correlate with fitness and explain 15-20 % of the variation in fitness. Correlations suggest that many populations have reduced fitness as a result of inbre eding depression and genetic drift. There is much fuss and concern thus, over genetic variation because of the fact that endangered species typically have lower levels of heterozygosity and the loss of adaptive genetic variation and inbreeding depression puts wildlife populations at an increased risk of extinction.Finally, this increase occurs as a result of the reduction of productive fitness because of inbreeding depression or due to the failure of tracking the change in abiotic and biotic environment of the population as a result of the loss of genetic variation through drift. DNA barcoding (Moritz & Cicero 2004) At the very core, the purpose of DNA barcoding is for large scale screening of one or a few reference genes in order to assign unknown individuals to species and enhance discovery of new species.In the hope of developing a comprehensive database of sequences that will serve as a comparison tool to sequences from sampled individuals, proponents used DNA barcoding. There i s, however, nothing new with DNA barcoding as it is an offshoot of the use molecular markers for the very same purpose except, in DNA barcoding, there is an increased scale and proposed standardization. The selection of one or more reference genes characterizes standardization, with regards to microbial community and in stimulating large scale phylogenetic analyses if of proven value, though whether or not one gene fits all remain to be a question.Presently, most methods of DNA barcoding are tree-based and can fall into two broadly defined classes. One class is the distance-based, wherein it is based on the degree of DNA sequence variation within and between species. This kind of approach converts DNA sequences into genetic distances and then uses these distances to establish identification schemes. It further defines a similarity threshold below which a DNA barcode is assigned to a known or a new species. There is also the mention by several authors of a â€Å"barcoding gap† , a distance-gap between intra- and interspecific sequences, for species identification.However, the distance-based approach seems to be ill suited as a general means for species identification and the discovery of new species. One reason is that substitution rates of mitochondrion DNA vary between and within species and between different groups of species. The varied substitution rates can result in broad overlaps of intra- and interspecific distances, and hinder the accurate assignment of query sequences. Another class which is the monophyly-based requires the recovery of species as discrete clades (monophyly) on a phylogenetic tree and is used to assign unknown taxa to a known or new species.Similarly, some issues complicate the use of monophyly in a barcoding framework. For example, the long-recognized problem of incomplete lineage sorting will yield gene genealogies that may differ in topology from locus to locus. The recently divergent taxa may not be reciprocally monophyletic due to lack of time needed to coalesce. In addition, the gene trees are not necessarily congruent with species trees, and the monophyly, while a discrete criterion is arbitrary with respect to taxonomic level.Moreover, there is a recently applied new technique that has been proposed as an alternative to tree-based approaches for DNA barcoding, the so called character-based DNA barcode method, which is based on the fundamental concept that members of a given taxonomic group share attributes that are absent from comparable groups. It is the kind of method that characterizes species through a unique combination of diagnostic characters rather than genetic distances. The four standard nucleotides (A,T,C,G) if found in fixed states in one species can be used as diagnostics for identifying that species.This way, species boundaries can be defined by a diagnostic set of characters which can be increased to any level of resolution by applying multiple genes. Presently, character-based DNA b arcode method has been proved useful for species identification and discovery of several taxa. In the view that single-gene sequence should be the primary identifier of species, a contention arises that if that is the case then there’ll be a real need to connect different life history stages and increase the precision and efficiency of field studies involving diverse and difficult-to-identify taxa.Although the DNA barcoding community has put emphasis on the importance of large-scale sequence database within the existing framework and practice of systematics, it should be bore in mind that DNA barcoding is not the primary answer in resolving the tree of life. Furthermore, as much as the term â€Å"DNA barcoding† appealing, it implies, however, that each species has a fixed and invariant characteristic. But this kind of implication renders unrest to the minds of evolutionary biologists.In evaluating thus, the promise and pitfall of DNA barcoding, two areas of application should be distinguished: the molecular diagnostics of individuals relative to described taxa and DNA-led discovery of new species. And although there is little doubt that large-scale and standardized sequencing, when integrated with existing taxonomic practice, can contribute significantly to the challenges of identifying individuals and increasing the rate of discovering biological diversity as presented by this study, the general utility of DNA barcoding still requires further scrutiny.PCR (Moore 2005) In rapidly copying a selected template sequence from a DNA mixture in vitro, PCR offers a wide range of applications such as sequence detection and isolation for research, forensics and species identification through the PCR itself and in combination with other techniques. PCR's new technique uses flourescent probes to monitor the amounted product at end of every cycle and PCR machines look for the cycle at which the can readily detect flourescence.PCR is also being used to monitor RNA through the addition of reverse transcriptase enzyme at the beginning to generate DNA template. In addition, there are now new applications of PCR like single nucleotide polymorphism detection and screening. Cytochrome Oxydase subunit 1 (COI) (Buhay 2009) COI plays a significant role in documenting biodiversity and remains to be the choice for phylogenetic and phylogeographic studies. COI is a mitochondrial protein-coding gene which is a widely accepted marker for molecular identification across diverse taxa.Mitochondrial DNA (mtDNA) have a relatively fast mutation rate, thus they result in significant differences between species. With respect to this, the mitochondrial cytochrome c oxidase subunit I (COI) gene with ~700bp was proposed to be a potential barcode or marker for molecular identification across various taxa. Furthermore, COI is a protein coding gene that has an open reading frame and in thecase of barcoding, COI can be highly divergent from the actual COI sequences which may cause major problems because species identification is based on sequence similarity.Pomacea canaliculata (Cowie 2002) The Pomacea canaliculata belongs to the family Ampullariidae. Its structure appears to have a slight dimorphism in shape of aperture and operculum. Females have broader mouth and concave operculum while convex in male. In terms of reproduction, oviposition often takes place at night or at early morning or evening about 24 hrs after copulation up to two weeks after mating (occurs three times per week) which occurs anytime of the day or night although there may be some diurnal rhythm.On each oviposition occasion a single clutch is laid of highly variable egg number. Moreover, the interval among successive ovipositions for p. canaliculata has been reported to be about five days and hatching generally takes place about two weeks after oviposition. The P. canaliculata breeds only during summer and grows into maturity in less than two months. P. canaliculata is s aid to be prolific and hence has rapid succession of generations which leads to rapid population expansion.They relatively inhabit still water and in water temperatures above 32 degree Celsius, it has been observed that the mortality of p. canaliculata is high. Whereas in low temperature p. canaliculata can survive 15-20 days at 0 degree Celsius, 2 days at -3 degree Celsius but only 6 hrs at -6 degree Celsius. And it is sufficiently tolerant of sea water to survive long enough to be carried by currents from one stream mouth to another, thereby expanding its distribution. P. canaliculata shows preferences among food plants.Its rate of growth has a direct correlation with its feeding on the preferred plant. Moreover, it is able to detect its food plants from some distance using chemical cues in the water. P. canaliculata, however, appears to be relatively generalist and indiscriminate that it is viewed to be particularly voracious compared to other Ampullariids. METHODOLOGY Sampling S nail samples were identified and collected from 2 countries in Asia, specifically in the Philippines and in China, where the P. canaliculata was introduced. In the Philippines; Los Banos (Dong et al. 011, p. 1778), 2 barangays in Tarlac (Brgy. Cabayaoasan, Paniqui and Brgy. Pance, Ramos) and Iloilo (Chichoco & Patdu 2012, p13), 44 snail samples were collected. And in China, specifically from Yuyao and Taizhou in Zhejiang province, Fuzhou in Fujian province, Guangzhou in Guangdong province, Nanning in Guangxi province, Kunming in Yunnan province, wherein a total of 120 samples were identified with the conserved sequence by Matsukura et al. (2008) and Pan et al. (2009) and then was collected (Dong et al. 2011, p. 1778).The snails were then stored, either by wrapping in paper, freezing or preserving it in ethanol, and brought into the respective labs in each country for the next steps; DNA extraction, PCR, electrophoresis and sequencing. DNA extraction The two studies used the phenol-c hloroform method (Bergallo et al. 2006) with an alternative of the Qiagen’s Dneasy extraction kit for China. The DNA concentration was determined spectrophotometrically and adjusted by a mini-gel method (Maniatis et al. , 1982) when the extracted DNA was enough, it was stored at 4oC to -20oC until needed. PCR and ElectrophoresisThe PCR method was basically done by choosing the right primers that will yield clearly reproduced bands and they tested the proper amounts and amplification effects of the components of PCR, which were the Mg2+, dNTP’s, DNA templates and polymerase, and the primers. After the mixture of the components and the DNA extracted, it was carried out in the thermocycler programmed for pre-denaturing at 94 °C for 3 min, followed by 26 cycles of 94 °C for 10-30s, 36-52 °C for 30-45s, extension of 65-72 °C for 60-90s, and the final extension for 5-7mins at 72 °C for final extension with 38-48 cycles.After which, the amplified products together w ith negative controls were run in electrophoresis to be separated and tested for contaminations, respectively. The products were then purified later on with the respective kits present in each lab. In the Philippines, the reaction was done with 2? L MgCl2, 5? L PCR buffer, 1? L dNTP, 2. 5 ? L of the primers, which were the LCO1490 and HCO2198, distilled H2O with 22. 75 ? L, 0. 25 Taq, and 10 ? L Q-buffer. The electrophoresis was done after the ethidium bromide staining (Maniatis et al. , 1982), analyzed through 1. % agarose gels and visualized under a transilluminator. In China, they made use of the ISSR-PCR analysis where they got four primers, which produced clearly reproduced bands, out of the 90 that was screened from the University of British Columbia’s primer set and the reactions were done with a volume of 20 ? l, containing 0. 2 mM of each dNTP, 1. 5 mM MgCl2, 0. 5 ? M primers, 1 U Taq polymerase and 10 ng DNA template, and also with the determination of the optimal r eaction system of ISSR for P. canaliculata (Dong et al. 2011, p. 1779).The products’ sizes after the amplification was estimated using DNA marker DL2000 and then was run in electrophoresis, which was done on 6% polyacrylamide gels, visualized by silver staining and then photographed (Li et al. , 2009). Sequencing/ Data analysis Chichioco and Patdu (2012) sent the DNA samples to the First Base Laboratory in Singapore for sequencing and the results were sent back to the DNA barcoding Laboratory after a week. The COI sequences were aligned in the BLAST, specifically the STADEN package version 1. 5. 3 and Bioedit Sequence Alignment Editor version 7. 0. 9. 0.Aside from the sequences sampled, other sequences and their haplotypes from the GenBank were also compared and matched. In Dong’s (2011) research, he made use of the RAPD fragments by labeling them into binary matrices, used them to get the similarity index, Sxy = 2nxy / nx+ ny, where nx and ny represent the number of R APD bands in individuals x and y, and nxy represents the number of shared bands between individuals, as stated by Nei and Li (1979), then averaging it across all the possible comparisons between individuals within a geographic sample to get the within samples similarity (Si).Between sample similarity corrected by within sample similarity Si and Sj of geographic samples i and j, respectively) is also calculated between pairs of individuals across samples i and j using the equation; S’ij = 1 + Sij – (Si and Sj)/2. Genetic distance between paired samples was then calculated as D’ij = 1- S’ij (Lynch, 1990). RESULTS AND DISCUSSION In the Philippines (Chichioco & Patdu 2012, p. 18-31) The collected samples from Brgy. Cabayaoasan were found in the elevated parts of a rice paddy, specifically, it was a muddy substrate with decaying leaves from the rice plants and surrounding trees while those that was found in Brgy.Pance was in the muddy bottom of the shallow fis h pond in the roots of water lilies and grasses. The samples from the two barangays in Tarlac and Iloilo had relatively different colors and sizes. Those that were collected from Brgy. Cabayaoasan had the largest size and they are colored black while those in Brgy. Pance had brown in color and still, those in Iloilo had very small sized specimens and some of the specimens can be mistaken as Pila conica snails if not examined properly. Primers affect the amplification success greatly, since according to Hajibabaei (2005) a 95% success is necessary for barcoding.The primers LCO1490 (SENSE) and HCO2198 are generally used for the amplification of forward and reverse fragments from COI genes. The DNA samples were subjected to the PCR and agarose gel electrophoresis (AGE), and they produced single discrete bands that suggest that the fragments were homogenous and start and end at the same point (Reece 2004). The bands that were brighter and distinct are more appropriate for sequencing bec ause it means that the DNA fragments were well amplified. The best DNA’s were chosen and forwarded to the First Base Laboratory in Singapore for sequencing.At the return of the results, other sequenced DNA barcodes were also collected and was aligned and compared with the Basic Local Alignment Search Tool (BLAST) database. Fig 1. Alignment of the COI gene sequences of the Pomacea canaliculata (CPT1-5 from Brgy. Cabayaoasan, PRT 7,9,10 from Brgy. Pance, IICK & IIPC1,3 from Iloilo) from the Philippines using Bioedit Sequence Alignment and ClustalW multiple Alignment (Chichioco & Patdu 2012, p. 26) By aligning the sequenced data, it can be seen that there are both similarities and differences among the genetic make-up of the samples.The differences are due to localized gap, mismatch and non-correspondence of bases along the COI fragments as pointed out in fig. 2 Fig 2. Comparisons of the COI sequences of the P. canaliculata samples from 35bp- 120bp (Chichioco & Patdu 2012, p. 28 ) As emphasized in fig. 2, on the 55bp-58bp, a sequence from CPT1 was observed having (5’-AATT-3’) while all the others have (5’-GGTA-3’). Even though this is a noticeable difference and could have been caused by mutation or variation, the difference is still low enough and less that 1% difference to be considered significant.But on the other hand, the PCa1 sample had 36 different base pairs compared with the others, which was 5. 5% difference and is high enough and can be considered significant since it is ;4% divergent(Meyer and Paulay 2005). Then with a ~98% confidence, it could be said that PCa1 is from an independent evolutionary lineage and might indicate a divergence within or outside its population or might have occurred due to relationships and interactions among the other species.The introduction of the P. canaliculata to different places may have an effect on its intra- and interpopulation and might be why it has various genetic sequences altho ugh it goes against the theory that introduced species becomes a founding population in a new habitat thus they have a limited gene pool and as a consequence genetic drift, which removes variability since it affects all genes, and bottleneck might occur, which reduces the new species to have a reduced genetic diversity.To observe the genetic variability, the sequences collected were compared with those from GenBank with the use of the BLAST software. As a result from 81 COI barcodes and 55 haplotypes, the samples collected showed 99% and 100% similarities with the different haplotypes thus it showed that the species has a high diversity within the populations. The phylogeography within and among the species does not apply on the P. analiculata since intra- and interpopulation diversity was observed which was shown by the multiple introduction throughout the Philippines, hence the different times of the introduction contributed more to its diversity and it coincides with the migrant pool model that says that the introduced population acquires more genetic variability because of the multiple sources of genetically divergent populations as compared to that of the local species (Slatkin 1997, Sakal et al 2001). In China (Dong et al 2010) The chosen primers an average of 124. bands, since they generated a total of 498 bands, which ranged from 150-2500bp and qualifies them for barcoding, as seen in table 1. Among the 140 individuals, 435 bands were polymorphic which was different for each primer. In table 2, Nei’s gene diversity (H) varied between 0. 2612 and 0. 3340, with an average of 0. 3044, and arranged in a descending order the populations, LB ; KM ; NN ; FZ ; TZ ; GZ ; YY while the Shannon’s information index (I) ranged from 0. 3910 to 0. 4856, with an average of 0. 4499.At the species level, the values of Nei’s and Shannon’s showed the same trend as that of PPB. AMOVA analysis showed that there are highly significant (P ; 0. 001) genetic differences among the seven populations of P. canaliculata. The genetic diversity was mostly due to the differences within the population (92. 76%) while the rest was due to among populations. The analysis tells the same as that of the Nei’s and Shannon’s information, which says that there was a relatively high level of genetic differentiation among populations. CONCLUSIONGenetics of different species are studied by means of DNA barcoding, mostly of the COI gene in the mitochondria, to know the taxon of a species and to understand their trends and characteristics not only morphologically but also genetically. The diversity of a species can also be tested by means of DNA barcoding as seen in the study of the Pomacea canaliculata. The P. canaliculata was introduced in Asia for agricultural purposes and was seen for its benefits but not its drawbacks, which later on resulted to it being invasive and a pest for both humans and other species.To understand the P. can aliculata further, its diversity was studied by means of DNA barcoding and was analyzed both qualitatively and quantitatively in the Philippines and China, respectively. Both the analysis showed the same outcome, wherein the results showed high levels of genetic diversity among populations. Because invasive species tends to give a negative feedback to those species in the local area, it is important to understand these alien species and to know how diverse they are so that proper management of these species could be done. REFERENCES Barker, G.M. 2002. Molluscs as Crop Pests. UK: CABI Publishing. (pp. 147-182) Buhay, Jennifer E. 2009. ‘‘COI-like’’ Sequences are Becoming Problematic in Molecular Systematic and DNA Barcoding Studies. Journal of Crustacean Biology 29(1): 96–110 Burns, George W. , Bottino, Paul J. 1989. The science of genetics 6th ed. New York: Macmillan Publishing Company. Chichioco, Monique Faye & Patdu, Edel Marie. 2012. DNA barcoding of Pomacea canaliculata and Pila conica using cytochrome c oxidase 1 (CO1) and small subunit (ssu) ribosomal (r) RNA barcodes (Undergraduate thesis).Main Library, University of the Philippines Baguio, Baguio City. Cowie, R. H. 2002. Apple snails (Ampullariidae) as agricultural pests: their biology, impacts and management. In: Molluscs as Crop Pests (ed. G. M. Barker), p. 145-192. CABI Publishing, Wallingford. Dodson, Edward O. 1956. Genetics: The Modern Science of Heredity. Philadelphia: W. B. Saunders Company. Dong, S. , Shentu, X. , Pan, Y. , Yu, X. , Wang, H. 2011. Evaluation of genetic diversity in the golden apple snail, Pomacea canaliculata (Lamarck), from different geographical populations in China by inter simple sequence repeat (ISSR).African Journal of Biotechnology Vol. 10(10), 1777-1783. Mendoza, Monica P & Serra, Karen Mae S. 2011. Mitochondrial DNA barcoding of Pomacea canaliculata and Pila conica (Undergraduate thesis). Main Library, University of the Philippines Bagu io, Baguio City. Moore, Pete. 2005. PCR: Replicating Success. Nature 435, 235-238. Moritz, C. , and Cicero,C.. 2004. DNA barcoding: promise and pitfalls. PLoS Biology 2(10), e354, 1529-1531. Reed, D. H. , Frankham, R. 2005. Correlation between Fitness and Genetic Diversity.Conservation Biology. Conservation Biology 17(1), 230-237. Wang, Xu-Mei. 2010. Optimization of DNA isolation, ISSR-PCR system and primers screening of genuine species of rhubarb, an important herbal medicine in China. Journal of Medicinal Plants Research 4(10), 904-908. Zou, S. , Li, Q. , Kong, L. , Yu, H. , Zheng, X. 2011. Comparing the Usefulness of Distance, Monophyly and Character-Based DNA Barcoding Methods in Species Identification: A Case Study of Neogastropoda. PLoS ONE 4(10), e26619.

Saturday, November 9, 2019

HIPAA THE IMPACT ON HEALTHCARE essays

HIPAA THE IMPACT ON HEALTHCARE essays Many different things have impacted the medical community in recent years including technological changes that have facilitated greater communications and medical discoveries. From a legislative perspective, the enactment of HIPPA rules and regulations by far is one of the most impactful. According to the American Medical Association (AMA), HIPPA "brings the most sweeping changes to healthcare since the creation of Before discussing the changes it is first critical to analyze what exactly HIPPA is. HIPPA stands for the Health Insurance Portability and Accountability Act of 1996, also known as Public Law 104-191 (HIPPA Group, 2002). HIPPA has accomplished the following related to healthcare:  ª Ensures access to health insurance, and also guarantees portability  ª Provides continuity of healthcare insurance coverage and prevents and strictly prohibits discrimination  ª Works to protect confidential health information and medical records  ª Enables patients to have more control over their records  ª Self employed persons are allowed health insurance tax deductions  ª Provides for better healthcare efficiencies HIPPA has affected the healthcare industry in a variety of ways. One manner in which it has impacted health providers is in the way they conduct business electronically. E based business and services are becoming increasingly popular in part due to the internet. Healthcare organizations can now share information about patients, store records and share insight related to patient conditions via electronic communications. As anyone knows however, due to security issues and ease of hacker access on the internet, it has become increasingly important that healthcare transactions be monitored from a safety and privacy perspective. HIPPA legisl...

Wednesday, November 6, 2019

When the Was Titanic Found

When the Was Titanic Found After the sinking of the Titanic on April 15, 1912, the great ship slumbered on the floor of the Atlantic Ocean for over 70 years before its wreckage was discovered. On September 1, 1985, a joint American-French expedition, headed by famous American oceanographer Dr. Robert Ballard, found the Titanic over two miles below the ocean’s surface by using an unmanned submersible called Argo. This discovery gave new meaning to the Titanic’s sinking and gave birth to new dreams in ocean exploration. The Titanic’s Journey Built in Ireland from 1909 to 1912 on behalf of the British-owned White Star Line, the Titanic officially left the European port of Queenstown, Ireland, on April 11, 1912. Carrying over 2,200 passengers and crew, the great ship  began its maiden voyage across the Atlantic, headed for New York. The Titanic carried passengers from all walks of life. Tickets were sold to first-, second-, and third-class passengers- the latter group largely consisting of immigrants seeking a better life in the United States. Famous first-class passengers included J. Bruce Ismay, the managing director of the White Star Line; business magnate Benjamin Guggenheim; and members of the Astor and Strauss families. The Sinking of the Titanic Only three days after setting sail, the Titanic struck an iceberg at 11:40 p.m. on April 14, 1912, somewhere in the North Atlantic. Although it took the ship over two and a half hours to sink, the vast majority of the crew and passengers perished due to a significant lack of lifeboats and improper use of those that did exist. The lifeboats could have held over 1,100 people, but only 705 passengers  were saved; nearly 1,500 perished the night the Titanic sank. People around the world were shocked when they heard that the â€Å"unsinkable† Titanic had sunk. They wanted to know the details of the disaster. Yet, however much the survivors could share, theories about how and why the Titanic sank would remain unsubstantiated until the wreckage of the great ship could be found. There was just one problem- no one was sure exactly where the Titanic had sunk. An Oceanographers Pursuit For as long as he could remember, Robert Ballard had wanted to find the wreckage of the Titanic. His  childhood in San Diego, California, near the water sparked his life-long fascination with the ocean, and he learned to scuba dive as soon as he was able. After graduating from the University of California, Santa Barbara in 1965 with degrees in both chemistry and geology, Ballard signed up for the Army. Two years later, in 1967, Ballard transferred to the Navy, where he was assigned to the Deep Submergence Group at the Woods Hole Oceanographic Research Institution in Massachusetts, thus beginning his illustrious career with submersibles. By 1974, Ballard had received two doctoral degrees (marine geology and geophysics) from the University of Rhode Island and had spent a lot of time conducting deep-water dives in Alvin,  a manned submersible he helped design. During subsequent dives in 1977 and 1979 near the Galapagos Rift, Ballard helped discover hydrothermal vents, which led in turn to the discovery of  the amazing plants that grew around these vents. Scientific analysis of these plants led to the discovery of chemosynthesis, a process in which plants use chemical reactions rather than sunlight to get energy. However many shipwrecks Ballard explored and however much of the ocean floor he mapped, Ballard never forgot about the Titanic. â€Å"I always wanted to find the Titanic, Ballard has said.  That was a Mt. Everest in my world- one of those mountains that had never been climbed.†* Planning the Mission Ballard wasn’t the first to try to find the Titanic. Over the years, there had been several teams that  had set out to find the wreckage of the famous ship; three of them had been funded by millionaire oilman Jack Grimm. On his last expedition in 1982, Grimm had taken an underwater picture of what he believed to be a propeller from the Titanic; others believed it was only a rock. The hunt for the Titanic was to continue, this time with Ballard. But first, he needed funding. Given Ballards history with the U.S. Navy, he decided to ask them to fund his expedition. They agreed, but not because they had a vested interest in finding the long-lost ship. Instead, the Navy  wanted to use the technology Ballard would create to also help them find and investigate the wreckage of two nuclear submarines (the USS Thresher and the USS Scorpion) that had been mysteriously lost in the 1960s. Ballard’s search for the Titanic provided a nice cover story for the Navy, who wanted to keep their search for their lost submarines a secret from the Soviet Union. Amazingly, Ballard maintained the secrecy of his mission even as he built the technology and used it to find and explore the remains of the USS Thresher  and the remains of the USS Scorpion. While Ballard was investigating these wreckages, he learned more about debris fields, which would prove crucial in finding the  Titanic. Once his secret mission was complete, Ballard was able to focus on searching for the Titanic. However, he  now had only two weeks in which to do it. Locating the Titanic It was late August 1985 when Ballard finally began his search. He  had invited a French research team, led by Jean-Louis Michel, to join this expedition. Aboard the Navy’s oceanographic survey ship, the Knorr, Ballard and his team headed to the likely location of the Titanic’s resting place- 1,000 miles due east of Boston, Massachusetts. While previous expeditions had used close sweeps of the ocean floor to search for the Titanic, Ballard  decided to conduct mile-wide sweeps in order to cover more area. He was able to do this for two reasons. First, after examining the wreckage of the two submarines, he discovered that ocean currents often swept lighter pieces of the wreck downstream, thus leaving a long debris trail. Secondly, Ballard had engineered a new unmanned submersible (Argo) that could explore wider areas, dive deeper, stay underwater for many weeks, and deliver crisp and clear pictures of what it found. This meant that Ballard and his team could stay on board the Knorr and monitor the images taken from Argo, with the hopes that those images would capture small, man-made pieces of debris. The Knorr arrived in the area on August 22, 1985, and began sweeps of the area using Argo. In the early morning hours of September 1, 1985, the first glimpse of the Titanic in 73 years appeared on Ballard’s screen. Exploring 12,000 feet below the ocean’s surface, the Argo relayed the image of one of the Titanic’s boilers embedded within the sandy surface of the ocean’s floor. The team on the Knorr was ecstatic about the discovery, although the realization that they were floating atop the graves of nearly 1,500 individuals lent a somber tone to their celebration. The expedition proved to be instrumental in shedding light on the Titanic’s sinking. Prior to the discovery of the wreckage, there was some belief that the Titanic had sunk in one piece. The 1985 images did not give researchers definitive information on the ship’s sinking; however, it did establish some basic foundations that countered early myths. Subsequent Expeditions Ballard returned to the Titanic in 1986 with new technology that allowed him to further explore the interior of the majestic ship. Images were collected that showed the remains of the beauty that so captivated those who had seen the Titanic at its height. The Grand Staircase, still-hanging chandeliers, and intricate  iron-work were all photographed during Ballard’s second successful expedition. Since 1985, there have been several dozen expeditions to the Titanic. Many of these expeditions have been controversial since salvagers brought up several thousand artifacts from the ship’s remains. Ballard has been widely outspoken against these efforts, claiming that he felt the ship deserved to rest in peace. During his two initial expeditions, he decided not to bring any discovered artifacts to the surface. He felt that others should honor the sanctity of the wreckage in a similar fashion. The most proliferate salvager of Titanic artifacts has been RMS Titanic Inc.  The company has brought many notable artifacts to the surface, including a large piece of the ship’s hull, passenger luggage, dinnerware, and even documents preserved in oxygen-starved compartments of steamer trunks. Due to negotiations between its predecessor company and the French government, the RMS Titanic group initially could not sell the artifacts, only put them on display and charge admission to recoup expenses and generate profit. The largest exhibition of these artifacts, over 5,500 pieces,  is located in Las Vegas, Nevada, at the Luxor Hotel, under the direction of the RMS Titanic Groups new name, Premier Exhibitions Inc. Titanic Returns to the Silver Screen Although the Titanic has been featured in numerous films through the years, it was James Cameron’s 1997 film, Titanic, that stimulated massive, worldwide interest in the ship’s fate. The movie became one of the most popular films ever made. The 100th Anniversary The 100th Anniversary of the sinking of the Titanic in 2012 also fueled renewed interest in the tragedy, 15 years after Cameron’s film. The wreckage site is now eligible to be named a protected area as a UNESCO World Heritage site, and Ballard is also working to preserve what remains. An expedition in August 2012  revealed that increased human activity has caused the ship to break down at a faster rate than previously expected. Ballard came up with a plan to slow the process of degradation- painting the Titanic while it remains 12,000 feet below the ocean’s surface- but the plan was never implemented.   The discovery of the Titanic was a momentous accomplishment,  but not only is the world conflicted about how to care for this historical wreck, but its existing artifacts could also now be in jeopardy. Premier Exhibitions Inc. filed for bankruptcy in 2016, asking permission from the bankruptcy court to sell the  Titanics artifacts. As of this publication, the court has not made a ruling on the request.

Monday, November 4, 2019

The History of Democracy in Algeria Research Paper

The History of Democracy in Algeria - Research Paper Example As the discussion stresses  colonization in Algeria began in the 5th century B. C. Native people of Algeria were forced to move back from the coastal areas by the successive waves of Roman, Byzantine, Turkish, Arab and French invaders. Among these Arab invaders have greatly influenced the natives of the country in terms of religion and language. However, Phoenicians were the first to invade the northern Algeria during 12th century B.C. Then the Romans came into the country and united the northern part of it. Under Roman rule Christianity flourished. However, it was ended by the Vandals, though a large section of area was still under the control of Berbers. Arabs started to dominate the area since 7th century.  This essay declares that  during World War I many Algerians were brought to the mainland of France for making them work in the French Army and various factories. After the World War I first organized movements began in the country for complete independence under the heads hip of Messali Hadj. Such movements started to gain momentum after World War II. Muslims were given some political rights in 1947 after a massive nationalist demonstration caused death of almost 88 Frenchmen and thousands of Muslims. In November, 1954 Algerians started their uprising in a way that could not be ignored by the French government. National Liberation Front (FLN) which was the main nationalist party introduced attacks on French people in both France and Algeria.   The war resulted in the fall of the government. In 1958 Charles de Gaulle came back in power and in 1962, Algeria’s independence was proclaimed (Phillip C. Naylor. ALGERIA - Part 3). History since Independence In 1963 Ahmed ben Bella took charge as Algeria’s first president. Under Bella’s rule, abandoned colonial holdings were nationalized. He supported various liberation movements in the country.

Saturday, November 2, 2019

Assignment s Example | Topics and Well Written Essays - 500 words

S - Assignment Example Thus, it would be more convenient for the defendants and plaintiffs to attend the court hearing in either Greece or Cyprus. According to Lear, the doctrine of forum non conveniens is supposed to provide convenience to the parties above all else (1147). In Piper vs Reyno the fact that most of the evidence including the plane wreckage and witnesses could be found in Scotland or near Scotland was given as one of the grounds for granting forum non conveniens. Similarly, the plane wreckage is in Athens and since the flights took off from Cyprus, further evidence could be obtained from the ground crew there. Furthermore, some of the evidence is in Greek and Greek law will be applicable in some matters. In Piper vs Reyno, it was reasoned that an American jury will have a hard time understanding foreign laws. If the case is heard in the district court, Greek law would have to apply and it is in doubt if the jury would comprehend Greek law. In Piper vs Reyno, American courts were cautioned against adjudicating foreign matters as this would place unnecessary burden on the American court system. In the case, both the defendant and plaintiffs are foreign citizens therefore meaning the US has little public interest in deciding the matter. In this case, the plaintiffs favor the district court as here they would obtain ten times as much compensation and damages for the suit. However, in Piper vs Reyno it was ruled that plaintiff’s favored forum had little bearing on granting dismissal on grounds of forum non conveniens. If courts were to take plaintiffs choice of forums as weighty consideration then dismissal would be rare. Furthermore, in Yavuz vs 61 MM LTD it was help that claims do not have to be similar to those offered by the district courts for a foreign jurisdiction to be accessed as an adequate forum In addition, the change of forum would not change either party’s case as plaintiffs could still make claims of